Molecular approach for analysis of model fungal genes encoding ligninolytic peroxidases in wood-decaying soil systems.
Identifieur interne : 000869 ( Main/Exploration ); précédent : 000868; suivant : 000870Molecular approach for analysis of model fungal genes encoding ligninolytic peroxidases in wood-decaying soil systems.
Auteurs : M. Stuardo [Chili] ; M. Vásquez ; R. Vicu A ; B. GonzálezSource :
- Letters in applied microbiology [ 0266-8254 ] ; 2004.
Descripteurs français
- KwdFr :
- ADN fongique (composition chimique), ADN fongique (isolement et purification), Analyse de séquence d'ADN (MeSH), Basidiomycota (enzymologie), Basidiomycota (génétique), Basidiomycota (isolement et purification), Basidiomycota (métabolisme), Gènes fongiques (MeSH), Microbiologie du sol (MeSH), Peroxidases (génétique), Protéines fongiques (génétique), Réaction de polymérisation en chaîne (méthodes).
- MESH :
- composition chimique : ADN fongique.
- enzymologie : Basidiomycota.
- génétique : Basidiomycota, Peroxidases, Protéines fongiques.
- isolement et purification : ADN fongique, Basidiomycota.
- métabolisme : Basidiomycota.
- méthodes : Réaction de polymérisation en chaîne.
- Analyse de séquence d'ADN, Gènes fongiques, Microbiologie du sol.
English descriptors
- KwdEn :
- Basidiomycota (enzymology), Basidiomycota (genetics), Basidiomycota (isolation & purification), Basidiomycota (metabolism), DNA, Fungal (chemistry), DNA, Fungal (isolation & purification), Fungal Proteins (genetics), Genes, Fungal (MeSH), Peroxidases (genetics), Polymerase Chain Reaction (methods), Sequence Analysis, DNA (MeSH), Soil Microbiology (MeSH).
- MESH :
- chemical , chemistry : DNA, Fungal.
- enzymology : Basidiomycota.
- genetics : Basidiomycota, Fungal Proteins, Peroxidases.
- isolation & purification : Basidiomycota, DNA, Fungal.
- metabolism : Basidiomycota.
- methods : Polymerase Chain Reaction.
- Genes, Fungal, Sequence Analysis, DNA, Soil Microbiology.
Abstract
AIMS
Test the use of nondegenerated consensus polymerase chain reaction (PCR) primers targeting lip and mnp sequences to detect ligninolytic fungi in wood-decaying soil systems, avoiding the need for enrichment or isolation on traditional fungal media culture.
METHODS AND RESULTS
The PCR primers were tested with total DNA isolated from incubations of wood-soil systems inoculated or not with the white-rot fungi Phanerochaete chrysosporium, or a white-rot sample obtained from a Nothofagus forest. The PCR products for lip and mnp sequences were only obtained in soil with P. chrysosporium-colonized wood chips. In these soil samples, reverse transcription-PCR analysis of lip and mnp PCR products indicated expression of LipA, LipB, LipJ and MnP isoenzymes.
SIGNIFICANCE AND IMPACT OF THE STUDY
This is the first assessment of the use of consensus PCR primers for direct detection of ligninolytic peroxidase genes in wood-decaying soil systems.
DOI: 10.1046/j.1472-765x.2003.01442.x
PubMed: 14687214
Affiliations:
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The PCR products for lip and mnp sequences were only obtained in soil with P. chrysosporium-colonized wood chips. In these soil samples, reverse transcription-PCR analysis of lip and mnp PCR products indicated expression of LipA, LipB, LipJ and MnP isoenzymes.</p></div><div type="abstract" xml:lang="en"><p><b>SIGNIFICANCE AND IMPACT OF THE STUDY</b></p><p>This is the first assessment of the use of consensus PCR primers for direct detection of ligninolytic peroxidase genes in wood-decaying soil systems.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">14687214</PMID><DateCompleted><Year>2004</Year><Month>04</Month><Day>02</Day></DateCompleted><DateRevised><Year>2019</Year><Month>09</Month><Day>17</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0266-8254</ISSN><JournalIssue CitedMedium="Print"><Volume>38</Volume><Issue>1</Issue><PubDate><Year>2004</Year></PubDate></JournalIssue><Title>Letters in applied microbiology</Title><ISOAbbreviation>Lett Appl Microbiol</ISOAbbreviation></Journal><ArticleTitle>Molecular approach for analysis of model fungal genes encoding ligninolytic peroxidases in wood-decaying soil systems.</ArticleTitle><Pagination><MedlinePgn>43-9</MedlinePgn></Pagination><Abstract><AbstractText Label="AIMS" NlmCategory="OBJECTIVE">Test the use of nondegenerated consensus polymerase chain reaction (PCR) primers targeting lip and mnp sequences to detect ligninolytic fungi in wood-decaying soil systems, avoiding the need for enrichment or isolation on traditional fungal media culture.</AbstractText><AbstractText Label="METHODS AND RESULTS" NlmCategory="RESULTS">The PCR primers were tested with total DNA isolated from incubations of wood-soil systems inoculated or not with the white-rot fungi Phanerochaete chrysosporium, or a white-rot sample obtained from a Nothofagus forest. The PCR products for lip and mnp sequences were only obtained in soil with P. chrysosporium-colonized wood chips. In these soil samples, reverse transcription-PCR analysis of lip and mnp PCR products indicated expression of LipA, LipB, LipJ and MnP isoenzymes.</AbstractText><AbstractText Label="SIGNIFICANCE AND IMPACT OF THE STUDY" NlmCategory="CONCLUSIONS">This is the first assessment of the use of consensus PCR primers for direct detection of ligninolytic peroxidase genes in wood-decaying soil systems.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Stuardo</LastName><ForeName>M</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, P. 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González</name><name sortKey="Vasquez, M" sort="Vasquez, M" uniqKey="Vasquez M" first="M" last="Vásquez">M. Vásquez</name><name sortKey="Vicu A, R" sort="Vicu A, R" uniqKey="Vicu A R" first="R" last="Vicu A">R. Vicu A</name></noCountry><country name="Chili"><noRegion><name sortKey="Stuardo, M" sort="Stuardo, M" uniqKey="Stuardo M" first="M" last="Stuardo">M. Stuardo</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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